Effect of Exercise Training on Some Anti-Inflammatory Adipokines, High Sensitivity C-Reactive Protein, and Clinical Outcomes in Sedentary Adults With Metabolic Syndrome.

OBJECTIVE: This study aimed to investigate the effects of aerobic interval training and resistance training on anti-inflammatory adipokines, high sensitivity C-reactive protein, and clinical outcomes in sedentary men with metabolic syndrome. METHODS: A total of 33 sedentary men with metabolic syndrome (age: 46.2 ± 4.6 years; body mass index: 35.4 ± 1.9 kg.m2) were randomly assigned to one of 3 groups: aerobic interval training (n = 12), resistance training (n = 10), or control (n = 11). Participants in the exercise groups completed a 12-week training program, 3 sessions per week, while those in the control group maintained their sedentary lifestyle. The levels of high sensitivity C-reactive protein (hs-CRP), omentin-1, adiponectin, lipid profiles, blood pressure, glucose metabolism, body composition, and peak oxygen uptake (VO2peak) were measured at baseline and after the intervention. RESULTS: Both aerobic interval training and resistance training significantly improved the levels of omentin-1 and adiponectin, as well as reduced inflammation, as indicated by a decrease in hs-CRP levels. Exercise training also led to significant improvements in lipid profiles, blood pressure, glucose metabolism, and body composition. Specifically, the aerobic interval training group had significantly greater increases in high-density lipoprotein cholesterol and VO2peak, as well as greater reductions in low-density lipoprotein cholesterol, triglycerides, and total cholesterol compared to the resistance training group. CONCLUSION: Exercise training, particularly aerobic interval training and resistance training, can be an effective non-pharmacological intervention for managing inflammation and improving cardiovascular health in metabolic syndrome patients.

The simultaneous occurrence of paraganglioma, Takotsubo syndrome, and Markis type I coronary artery ectasia in the same patient is a rare, high-risk clinical syndrome: a case report.

BACKGROUND: Population-wide, paraganglioma (PGL) is uncommon. The incidence of Takotsubo syndrome (TTS) ranges from 0.5% to 0.9% and also is an exceedingly rare manifestation of PGL. Coronary artery ectasia (CAE) is also uncommon, with an incidence ranging from 1.2% to 4.9%. Herein, we present a case of PGL, TTS, and Markis type I CAE that occured in the same patient. CASE PRESENTATION: A man in his early 40s was admitted to our hospital with a 16-hour history of abdominal colic. Computed tomography and laboratory examination led to the diagnosis of PGL, coronary angiography led to the diagnosis of Markis type I or Chinese type III CAE, and two echocardiographic examinations led to the diagnosis of TTS. When the patient was treated by phenoxybenzamine instead of surgery for the PGL, his blood pressure and glucose level gradually returned to normal. The CAE was treated by thrombolysis, antiplatelet medications, atorvastatin, and myocardial protection therapies. No symptoms of PGL, CAE, or TTS were seen during a 6-month follow-up, and the patient had an excellent quality of life. We confirmed that phenoxybenzamine was the cause of the TTS because paradoxical systolic motion of the apex, inferior wall, left ventricular anterior wall, and interventricular septum were similarly recovered when the PGL was treated by phenoxybenzamine. CONCLUSIONS: To raise awareness of this illness and prevent misdiagnosis, we have herein presented a case of TTS that was brought on by PGL with Markis type I CAE for clinicians' reference. In addition, in clinical practice, we should consider the possibility of a concomitant coronary artery disease even if the TTS is caused by a PGL-induced catecholamine surge.

Comparison of 1064-nm Nd:YAG picosecond laser using fractional micro-lens array vs. ablative fractional 2940-nm Er:YAG laser for the treatment of atrophic acne scar in Asians: a 20-week prospective, randomized, split-face, controlled pilot study.

Background: The 1064-nm Nd:YAG picosecond lasers using fractional micro-lens array (P-MLA) was a promising therapy for skin resurfacing. However, no studies have compared P-MLA with ablative fractional 2940-nm Er:YAG lasers (AF-Er) in the treatment of atrophic acne scars. Objectives: To evaluate the efficacy and safety of P-MLA and AF-Er for the treatment of atrophic acne scars. Methods: We performed a prospective, randomized, split-face, controlled pilot study. Thirty-one Asian patients with mild to moderate atrophic acne scars underwent four consecutive sessions of randomized split-face treatment with P-MLA and AF-Fr at 4-week intervals. The efficacy of the two devices were evaluated by Echelle d'Evaluation Clinique des Cicatrices d'acne (ECCA) grading scale, Investigator's Global Assessment (IGA) score and patient's satisfaction. VISIA analysis was also performed to evaluate the pore and skin texture. Adverse events were recorded at each follow-up. Results: The P-MLA afforded comparable clinical responses in scar appearance as AF-Er based on the investigator's assessments (ECCA percent reduction: 39.11% vs. 43.73%; IGA score: 2.97 ± 0.65 vs. 3.16 ± 0.68; P > 0.05 for both). However, the result of patient satisfaction indicated the AF-Er-treated side achieved a slightly greater improvement in scar appearance (3.97 ± 0.78 vs. 3.55 ± 0.71; P < 0.05). Overall, the two devices did not differ largely in terms of efficacy. VISIA analysis revealed similar changing patterns of the pore and skin texture between two devices. For safety profiles, no serious side effects were reported on both sides. The P-MLA showed lower pain level, shortened duration of crust shed and edema, and less occurrence of PIH (P < 0.05 for all). Conclusion: Compared with AF-Er, P-MLA afforded comparable effect and more safety profiles in treating atrophic acne scars in Asian patients. Clinical trial registration: ClinicalTrials.gov, identifier NCT05686603.

The novel peptide PFAP1 promotes primordial follicle activation by binding to MCM5.

Premature ovarian failure (POF) is a complication of ovarian dysfunction resulting from the depletion or dysfunction of primordial follicles (PFs) in the ovaries. However, residual follicles that have the potential to be activated are present in POF or aged women. Little is known about the mechanisms by which the remaining dormant PFs in POF patients are activated. Using mass spectrometry, we screened differentially generated peptides extracted from the ovarian cortical tissue biopsies of patients with or without POF, during which we identified PFAP1, a peptide that significantly promoted the activation of PFs in the ovaries of 3 dpp mice in vitro. PFAP1 reversed age-related fertility damage in vivo to a certain extent, promoted estrogen (E2) and anti-mullerian hormone (AMH) production (p < .05), and decreased the levels of follicle-stimulating hormone (FSH) (p < .05). In newborn mouse ovaries, PFAP1 could bind to the protein minichromosome maintenance protein 5 (MCM5) and inhibit its ubiquitination and degradation. In addition, PFAP1 promoted the proliferation of GCs, probably by regulating the function and production of MCM5. In conclusion, PFAP1 could promote the activation of PFs in the ovaries of newborn mice, partially restore the ovarian function of aged mice, and increase the proliferation of primary granulosa cells (GCs) by regulating the function of MCM5. PFAP1 is a promising novel peptide that may be developed into a new therapeutic agent for POF and other ovarian diseases.

Polyurethane Elastomer Layered Nanocomposite Material for Sports Grounds and the Preparation Method Thereof.

Polyurethane, as a rubber material, can relieve the load on the ground and provide seismic design for the venue, which is of great significance for sports venues. In order to improve the seismic resistance and abrasion resistance of materials for sports fields and reduce accidents in sports, this article has carried out research on the polyurethane elastomer layered nanocomposites for sports fields and their preparation. Today's world is a challenging era of science and technology. The fields of biotechnology, information, medicine, energy, environment, and national defense and security are closely related to the development of high tech, and the requirements for materials are becoming increasingly diversified. Polymer nanocomposite coating has the dual characteristics of organic and inorganic components. It not only retains the advantages of a polymer but also endows it with versatility. It meets the current application needs. It is a hot spot in today's research. Among them, there are two major problems in the composite process of nanomaterials and polymers: dispersion and compatibility. How to improve the dispersion of nanoparticles and enhance the compatibility between nanoparticles and polymers is an urgent problem to be solved. In the method part, this article introduces a small amount of polyurethane and polyurethane elastomers formed after polyurethane modification and introduces layered compounds and nanocomposites and introduces several models involved in nanomaterials in terms of algorithms. In the analysis part, this paper conducts a comprehensive analysis of the hard segment mass fraction, mechanical properties, thermal decomposition behavior, degradation mechanism, and dynamic mechanical properties. With the increase of GO content, the tensile strength increases significantly and the elongation at break becomes smaller and smaller. When the GO content increases from 0% to 2%, the tensile properties of the WPU film increase from 2.6 MPa to 7.9 MPa and the fracture of the elongation decreased from 201.7% to 62.8%. This shows that the increase in GO content will make the composite material harder and brittle. It can be seen from the experimental results that the preparation of the polyurethane elastomer layered nanocomposite material designed in this paper has a good application effect on sports venues.

Regulation of electronic structures of MOF-derived carbon via ligand adjustment for enhanced Fenton-like reactions.

Peroxymonosulfate (PMS)-based Fenton-like reactions are widely used for wastewater remediation. Metal-free carbonaceous activators can avoid the secondary pollution caused by metal leaching but often suffer from insufficient activity due to limited active centers and mass transfer barriers. Here, we prepared a series of heteroatom (N, S, F)-doped, highly porous carbonaceous materials (UC-X, X = N, S, F) by pyrolyzing UiO-66 precursors assembled by various organic ligands. Density functional theory calculations showed that the heteroatoms modulated the electronic structures of the carbon plane. UC-X exhibited significantly enhanced PMS activation capability compared with the undoped counterpart, in the efficiency order of UC-N > UC-S > UC-F > UC. UC-N (calcined at 1000°C) showed the best PMS activation, exceeding that of commonly used carbocatalysts. The prominent performance of UC-N originated from its unique porous structure and homogeneously dispersed graphitic N moieties. Trapping experiments and electron spin resonance showed a nonradical degradation pathway in the UC-N/PMS system, through which organics were oxidized by donating electrons to UC-N/PMS* metastable complexes. This work not only reports a universal way to access high-performance, metal-free PMS activators but also provides insight into the underlying mechanism of the carbon-activated PMS process.

Cobalt-Enhanced Mass Transfer and Catalytic Production of Sulfate Radicals in MOF-Derived CeO2 • Co3 O4 Nanoflowers for Efficient Degradation of Antibiotics.

Antibiotics discharge has been a critical issue as the abuse in clinical disease treatment and aquaculture industry. Advanced oxidation process (AOPs) is regarded as a promising approach to degrade organic pollutants from wastewater, however, the catalysts for AOPs always present low activities, and uncontrollable porosities, thus hindering their further wider applications. In this work, an aliovalent-substitution strategy is employed in metal-organic framework (MOF) precursors assembly, aiming to introduce Co(II/III) into Ce-O clusters which could modify the structure of the clusters, then change the crystallization, enlarge the surface area, and regulate the morphology. The introduction of Co(II/III) also enlarges the pore size for mass transfer and enriches the active sites for the production of sulfate radicals (SO4• - ) in MOF-derived catalysts, leading to excellent performance in antibiotics removal. Significantly, the CeO2 •Co3 O4 nanoflowers could efficiently enhance the generation of sulfate radical SO4• - and promote the norfloxacin removal efficiency to 99% within 20 min. The CeO2 •Co3 O4 nanoflowers also present remarkable universality toward various antibiotics and organic pollutants. The aliovalent-substitution strategy is anticipated to find wide use in the exploration of high-performance MOF-derived catalysts for various applications.

Atomically Dispersed Cobalt Sites on Graphene as Efficient Periodate Activators for Selective Organic Pollutant Degradation.

Pollutant degradation via periodate (IO4-)-based advanced oxidation processes (AOPs) provides an economical, energy-efficient way for sustainable pollution control. Although single-atomic metal activation (SMA) can be exploited to optimize the pollution degradation process and understand the associated mechanisms governing IO4--based AOPs, studies on this topic are rare. Herein, we demonstrated the first instance of using SMA for IO4- analysis by employing atomically dispersed Co active sites supported by N-doped graphene (N-rGO-CoSA) activators. N-rGO-CoSA efficiently activated IO4- for organic pollutant degradation over a wide pH range without producing radical species. The IO4- species underwent stoichiometric decomposition to generate the iodate (IO3-) species. Whereas Co2+ and Co3O4 could not drive IO4- activation; the Co-N coordination sites exhibited high activation efficiency. The conductive graphene matrix reduced the contaminants/electron transport distance/resistance for these oxidation reactions and boosted the activation capacity by working in conjunction with metal centers. The N-rGO-CoSA/IO4- system exhibited a substrate-dependent reactivity that was not caused by iodyl (IO3·) radicals. Electrochemical experiments demonstrated that the N-rGO-CoSA/IO4- system decomposed organic pollutants via electron-transfer-mediated nonradical processes, where N-rGO-CoSA/periodate* metastable complexes were the predominant oxidants, thereby opening a new avenue for designing efficient IO4- activators for the selective oxidation of organic pollutants.

Current mechanisms of primordial follicle activation and new strategies for fertility preservation.

Premature ovarian insufficiency (POI) is characterized by symptoms caused by ovarian dysfunction in patients aged <40 years. It is associated with a shortened reproductive lifespan. The only effective treatment for patients who are eager to become pregnant is IVF/Embryo Transfer (ET) using oocytes donated by young women. However, the use of the technique is constrained by the limited supply of oocytes and ethical issues. Some patients with POI still have some residual follicles in the ovarian cortex, which are not regulated by gonadotropin. These follicles are dormant. Therefore, activating dormant primordial follicles (PFs) to obtain high-quality oocytes for assisted reproductive technology may bring new hope for patients with POI. Therefore, this study aimed to explore the factors related to PF activation, such as the intercellular signaling network, the internal microenvironment of the ovary and the environment of the organism. In addition, we discussed new strategies for fertility preservation, such as in vitro activation and stem cell transplantation.

Synergic removal of tetracycline using hydrophilic three-dimensional nitrogen-doped porous carbon embedded with copper oxide nanoparticles by coupling adsorption and photocatalytic oxidation processes.

Adsorption and photocatalytic oxidation are promising technologies for eliminating antibiotics (e.g. tetracycline) in aquatic environments. However, traditional powdery nanomaterials are limited by drawbacks of difficult separation and lack of synergistic function, which do not conform to the practical demand. Herein, we developed a simple one-step gelation-pyrolysis route to fabricate hydrophilic three-dimensional (3D) porous photocatalytic adsorbent, in which CuO nanoparticles are uniformly and firmly embedded in nitrogen-doped (N-doped) porous carbon frameworks. The obtained N-doped carbon/CuO bulky composites exhibited excellent ability to adsorb tetracycline hydrochloride (TC), which was subsequently photo-oxidized under visible light. Their hydrophilic nature favors the adsorption processes toward TC, with a maximum adsorption capacity reaching 25.03 mg∙g-1. In addition, >94.4% of TC molecules could be photo-degraded in 4 h with good cycling efficiency after three consecutive tests. Finally, a reaction scheme for removal process of TC was proposed. The obtained 3D porous N-doped carbon/CuO nanocomposites show great promise for efficient removal of antibiotics in aqueous solution by synergistically utilizing adsorption and photocatalytic oxidation processes.

Microwave-assisted hydrothermal assembly of 2D copper-porphyrin metal-organic frameworks for the removal of dyes and antibiotics from water.

Adsorption and photocatalysis are promising strategies to remove pollutants of dyes and antibiotics from wastewater. In this study, we demonstrate a rapid microwave-assisted hydrothermal route for the assembly of 2D copper-porphyrin Metal-Organic Frameworks (Cu-TCPP MOFs) within 1 h. The resulting 2D Cu-TCPP nanosheets with excellent crystallinity and a large surface area (342.72 m2/g) exhibited outstanding adsorption performance for typical dyes with adsorption capacities of about 185 mg/g for rhodamine B, 625 mg/g for methylene blue, and 290 mg/g for Congo red, respectively, as well as for representative antibiotics with adsorption capacities of about 130 mg/g for oxytocin, 150 mg/g for tetracycline, and 50 mg/g for norfloxacin, respectively. Meanwhile, the as-prepared 2D Cu-TCPP showed good photocatalytic degradation activity of pollutants after adsorption under irradiation by visible light, reaching removal efficiencies of 81.2 and 86.3% toward rhodamine B and norfloxacin, respectively. These results demonstrate the promising potential of 2D Cu-TCPP for use in the removal of contaminants from wastewater.

An all-in-one strategy for the adsorption of heavy metal ions and photodegradation of organic pollutants using steel slag-derived calcium silicate hydrate.

Low-cost and high-performance materials or techniques that could synergistically remove inorganic heavy metals and organic pollutants in a simple manner are highly desired. Herein, we report a simple and facile strategy by converting poisonous heavy metals into photocatalyst for the in-situ photodegradation of organic pollutants employing steel slag-derived calcium silicate hydrate (CSH). The CSH was synthesized by alkali activation method and showed hierarchical structure and amorphous phase. And, the material exhibited excellent adsorption performance towards all selected heavy metals. After adsorption, the heavy metals were converted into the corresponding amorphous metal hydroxides on the surface of CSH. The resulting CSH-supported amorphous metal hydroxides can act as visible-light photocatalysts for the photodegradation of organic pollutants. The optimal results for the whole water purification route using CSH are > 100 mg/g adsorption capacity for Cu2+ and ˜63% / 8 h photodegradation efficiency for methylene blue under visible light. The total cost for the whole route is < 0.1 $/g pollutants, much lower than traditional technologies. The strategy using steel slag derived-CSH not only meets the requirements for high-performance and low-cost materials, but also resolves the challenging issues of developing an all-in-one treatment for heavy metal ions and organic pollutants, which will be of great significance to wastewater purification.

Multifunctional Mineral Hydrogels: Potential in Artificially Intelligent Skins and Drug Delivery.

Hydrogels have received considerable attention due to their potential applications in the fields of drug delivery, tissue engineering, and stimuli-responsive devices. Nonetheless, it is still a great difficulty in designing hydrogels with multifunctional characteristics including excellent antibacterial activity and appropriate mechanical and remarkable sensing properties. In the present study, a novel type of organic-inorganic adhesive is demonstrated, which comprises inorganic matter of amorphous calcium phosphate particles and organic substances of poly(acrylic acid) and chitosan. The hydrogel possesses excellent biocompatible and antibacterial activity, unique viscoelastic properties, high quantity of drug load, and remarkably sensitive pressure sensing, which have potential use as antibacterial biomaterials, artificially intelligent skins, and drug delivery carriers.

A fully chimeric IgG antibody for ROR1 suppresses ovarian cancer growth in vitro and in vivo.

BACKGROUND: Over-expression of Receptor-tyrosine-kinase-like Orphan Receptor 1 (ROR1) in cancer cells has been reported in the context of several tumors (including ovarian cancer) and is associated with poor prognosis. The aim of this study was to construct a fully chimeric anti-ROR1 IgG antibody (ROR1-IgG) and investigate its antitumor activity against ovarian cancer cells, bothin vitro and in vivo. METHODS: A fully chimeric anti-ROR1 IgG antibody (ROR1-IgG) eukaryotic expression vector was constructed and ROR1-IgG antibody was expressed in CHO cells. The characteristics of ROR1-IgG were investigated by ELISA, SPR, Western blotting, FACS and fluorescence staining analyses. CCK8 and wound healing assays were performed to determine inhibition and migration capacity of ovarian cancer cells after treatment with ROR1-IgGin vitro. Further, the antitumor activity of ROR1-IgG was assessed in vivo using tumor-mice xenograft model. RESULTS: The results showed that ROR1-IgG could specifically bind to ROR1-positive cells (HO8910 and A2780) with a high affinity. Functional studies revealed that ROR1-IgG inhibited the malignant behavior of ROR1-positive cells (HO8910 and A2780) in a time- and dose-dependent manner. These effects were not observed in ROR1-negative lose386 cells. The tumor inhibition rates following treatment with low, medium, and high concentrations of ROR1-IgG were approximately 47.72%, 53.79%, and 60.51%, respectively. In addition, the expression of Bcl-2 was obviously reduced while that of Bax was distinctly elevated in xenografts. CONCLUSIONS: Collectively, our findings suggest that ROR1-IgG may be a novel therapeutic agent for patients with ROR1-positive ovarian cancer.

Self-templated microwave-assisted hydrothermal synthesis of two-dimensional holey hydroxyapatite nanosheets for efficient heavy metal removal.

Heavy metals have caused serious environmental problems and threat to human health. Ultrathin and holey two-dimensional (2D) nanosheets have recently drawn significant attention as superb adsorbent material to remove heavy metal ions due to their unique physicochemical properties. Herein, we report a self-template-directed ultrafast reaction route to synthesis porous hydroxyapatite (Ca10(PO4)6(OH)2) nanosheets via a microwave-assisted hydrothermal method using poly(allylamine hydrochloride) as an additive. The resulting hydroxyapatite nanosheets showed a high specific surface area (92.9 m2 g-1) and excellent adsorption performance for various heavy metal ions including Pb(II), Cu(II), and Cd(II), with maximum adsorption capacities of 210.5, 31.6, and 24.9 mg g-1, respectively. The adsorption kinetics fitted well with the pseudo-second-order equation and the equilibrium data showed a high correlation coefficient with the Langmuir model. Based on the experimental results and analysis, we can conclude that the sorption of heavy metal ions with the hydroxyapatite nanosheets mainly attributes to surface complexation and cation exchange. The present synthetic strategy allows the fast and massive production of porous hydroxyapatite ultrathin nanosheets and may also potentially be applicable to the fabrication of other metal phosphates with assembled or hierarchical porous structures towards various applications such as water purification.

Multifunctional Nanoengineered Hydrogels Consisting of Black Phosphorus Nanosheets Upregulate Bone Formation.

Tissue-engineered hydrogels have received extensive attention as their mechanical properties, chemical compositions, and biological signals can be dynamically modified for mimicking extracellular matrices (ECM). Herein, the synthesis of novel double network (DN) hydrogels with tunable mechanical properties using combinatorial screening methods is reported. Furthermore, nanoengineered (NE) hydrogels are constructed by addition of ultrathin 2D black phosphorus (BP) nanosheets to the DN hydrogels with multiple functions for mimicking the ECM microenvironment to induce tissue regeneration. Notably, it is found that the BP nanosheets exhibit intrinsic properties for induced CaP crystal particle formation and therefore improve the mineralization ability of NE hydrogels. Finally, in vitro and in vivo data demonstrate that the BP nanosheets, mineralized CaP crystal nanoparticles, and excellent mechanical properties provide a favorable ECM microenvironment to mediate greater osteogenic cell differentiation and bone regeneration. Consequently, the combination of bioactive chemical materials and excellent mechanical stimuli of NE hydrogels inspire novel engineering strategies for bone-tissue regeneration.

Luffa sponge-derived hierarchical meso/macroporous boron nitride fibers as superior sorbents for heavy metal sequestration.

Boron nitride (BN) has received tremendous attention as a promising adsorbent material. However, unsatisfactory uptake capacities over heavy metal ions limit their practical applications. Herein, we have synthesized a novel hierarchical meso/macroporous BN fibers (MBNFs) via a simple carbothermal reduction method using luffa sponge as a template. The as-obtained MBNFs comprise densely arranged parallel macrochannels on a micrometer scale, with mesopores on the surface of the channel. The resulting MBNFs exhibited remarkable adsorption performance for different heavy metal ions including Cd2+, Zn2+, Cr3+, and Pb2+ with maximum uptake capacities as high as 2989, 1885, 723, and 453 mg/g, respectively. In particular, the adsorption capacity for Cd2+ and Zn2+ exceed the highest values reported for BN materials. In addition, the MBNFs showed excellent stability to re-use for a few times. The present MBNFs materials prepared using cheap and earth abundant luffa sponge may find broad applications such as adsorbent for environmental remediation applications.

Few-layer MoS2 nanosheet-coated KNbO3 nanowire heterostructures: piezo-photocatalytic effect enhanced hydrogen production and organic pollutant degradation.

The introduction of a piezoelectric field has been considered as a promising strategy to enhance photocatalytic activity by inhibiting the recombination of photogenerated electron-hole pairs in semiconductor photocatalysts. In this work, a novel heterostructured photocatalyst that combines piezoelectric KNbO3 nanowires and few-layer MoS2 nanosheets was designed and synthesized via a simple two-step hydrothermal method. Under simulated solar light illumination, the KNbO3/MoS2 heterostructures showed significantly enhanced photocatalytic H2 production and organic pollutant (e.g. rhodamine B) degradation efficiency, compared to pristine KNbO3 nanowires and MoS2 nanosheets. The photocatalytic activity can be further improved greatly by co-utilizing the solar and mechanical energy provided by ultrasonic vibration. The enhancement of photocatalytic activity can be attributed to the promotion of charge separation caused by the synergetic effect of the formation of a heterojunction and the internal piezoelectric field induced by mechanical vibration. Our findings may provide insight into strategies for designing highly efficient piezoelectric material-based nanocomposites for various photocatalytic applications such as environmental remediation and renewable energy production.

A novel human monoclonal Trop2-IgG antibody inhibits ovarian cancer growth in vitro and in vivo.

Trop2 is a tumor-related antigen closely related to the development of a variety of tumors and has been identified as a promising target for cancer immunotherapy. In this study, a Trop2-IgG antibody was constructed by a eukaryotic expression system based on our previously constructed Trop2-Fab antibody. SDS-PAGE, cell ELISA, affinity assays, fluorescence staining and FACS analyses were performed to characterize Trop2-IgG. Then, CCK-8, wound healing, Transwell and annexin V-PI assays were employed to evaluate the tumor inhibitory effects of Trop2-IgG on OC in vitro, while tumor-bearing mice were constructed to examine the tumor inhibitory effects of Trop2-IgG on OC in vivo. Trop2-IgG was successfully constructed by a eukaryotic expression system and maintained recognition characteristics to Trop2 antigen. In vitro, Trop2-IgG could inhibited tumor cell growth, migration, and invasion compared to those of control cells and induced tumor cell apoptosis. In vivo, Trop2-IgG exerted critical tumor inhibitory effects in OC xenografts. Our data suggest that the use of Trop2-IgG provides a potential therapeutic strategy for the immunotherapy of Trop2-expressing OC.